INTEGRAL ESTIMATION OF OXIDATIVE STATUS IN PATIENTS WITH ACUTE TOXIC HEPATITIS AND CHRONIC ALCOHOLIC LIVER DISEASE
Abstract
BACKGROUND Acute toxic hepatitis associated with acute poisoning with alcohol-containing disinfectants remains a medical and social problem.
MATERIAL AND METHODS With an aid of chemiluminescence, we performed the integrated assessment of the oxidative status in the blood serum and homogenized liver biopsy tissue of 62 patients with the diagnosis «severe acute toxic hepatitis associated with the use of alcohol-containing disinfectants».
RESULTS The research showed that at the onset of a disease, patients with acute toxic hepatitis had an expressed activation of free radical oxidation of the blood serum and biopsy tissue. This was indicated by almost double increase in the intensity of free radicals generation (Ssp). This signifi cantly increased production of peroxide (Sind-1) and hydroxyl radicals (Slum) — 2.5 and 1.86 times, respectively; also, it increased concentration of lipid hydroperoxides (h) almost three times, evidencing activation of the initial stage of lipid peroxidation There was no statistically signifi cant fall of figures indicating the liver parenchymal oxidative status 30 days after the admission. The same situation was observed 6 months after the beginning of the study.
CONCLUSION Analyzing chemiluminescence scans of blood serums up to 30 days from admission, it is possible to conclude indirectly on a condition of the oxidative status in a liver parenchyma of patients.
About the Authors
A. Y. Shchupak
Far-eastern State Medical University, Ministry of Health of the Russian Federation, Khabarovsk, Russian Federation
Russian Federation
Russian Federation
O. A. Lebedko
Far-eastern State Medical University, Ministry of Health of the Russian Federation, Khabarovsk, Russian Federation
Russian Federation
Russian Federation
V. V. Yukhno
Far-eastern State Medical University, Ministry of Health of the Russian Federation, Khabarovsk, Russian Federation
Russian Federation
Russian Federation
References
1. Alekseenko S.A., Shchupak A.Yu., Lebed’ko O.A., Puchkov Yu.B. Otsenka effektivnosti kompleksnoy terapii toksicheskogo gepatita vsledstvie upotrebleniya spirtosoderzhashchikh dezinfektantov [Evaluation of effectiveness of complex therapy of toxic hepatitis due to the use of alcohol-containing disinfectants]. Sibirskiy meditsinskiy zhurnal. 2008; 6: 58–63. (In Russian).
2. Bonitenko E.Yu., Petrov A.N., Shevchuk M.K. Vliyanie poligeksametilenguanidina gidrokhlorida na toksichnost’ etilovogo spirta [The influence of polyhexamethylenguanidine hydrochloride toxicity ethyl alcohol]. Tezisy Rossiyskoy nauch.-prakt. konf. [Abstracts of the Russian scientific.-practical. Conf.] Yekaterinburg, September 25–26, 2008. 100–102. (In Russian).
3. Alekseenko S.A., Shchupak A.Yu., Lebed’ko O.A., Puchkov Yu.B. Vliyanie ursosana na klinicheskuyu simptomatiku, morfologicheskie izmeneniya v pecheni i pokazateli oksidativnogo statusa pri gepatotoksicheskikh porazheniyakh vsledstvie upotrebleniya spirtsoderzhashchikh dezinfektantov [Effect of Ursosan on clinical symptomatology, morphological changes in the liver and indicators of oxidative status in hepatotoxic lesions by the use of alcohol-containing disinfectants]. Klinicheskie perspektivy gastroenterologii, gepatologii. 2009; 2: 18–23. (In Russian).
4. Ivashkin V.T., Bueverov A.O. Toksicheskiy gepatit, vyzvannyy otravleniem surrogatami alkogolya [Toxic hepatitis caused by poisoning with alcohol surrogates]. Rossiyskiy zhurnal gastroenterologii, gepatologii, koloproktologii. 2007; 1: 4-8. (In Russian).
5. Shchupak A.Yu., Alekseenko S.A., Yukhno V.V. Ostryy toksicheskiy gepatit, razvivshiysya vsledstvie upotrebleniya spirtosoderzhashchikh dezinfektantov: metod. posobie [Acute toxic hepatitis, which developed as a result of the use of alcohol-based disinfectants]. Khabarovsk, 2008. 15 p. (In Russian).
6. Lebed’ko, O.A., Timoshin S.S. Aktivnye kislorodnye metabolity kak universal’nye messendzhery protsessov signal’noy transduktsii [Active oxygen metabolites as a universal messenger processes of signal transduction]. Dal’nevostochnyy meditsinskiy zhurnal. 2004; 4: 95–98. (In Russian).
7. Babior B.M. Phagocytes and oxidative stress. Am J Med. 2000; 109 (1): 33–44.
8. Tomilka G.S., Shchepilova O.V., Lebed’ko O.A. Otsenka svobodnoradikal’nogo statusa syvorotki krovi bol’nykh virusnym gepatitom A legkoy i srednetyazheloy formami [Evaluation of free radical status in the blood serum of patients with the light and moderate forms of the viral hepatitis A]. Dal’nevostochnyy meditsinskiy zhurnal. 2007; 4: 38–39. (In Russian).
9. Artyukhov V.G., Andreeshcheva E.M., Popova T.N., Matasova L.V. Osobennosti svobodnoradikal’nogo okisleniya i kataliticheskie svoystva akonitatgidratazy v pecheni krys v norme i pri toksicheskom gepatite [Features of free radical oxidation and catalytic properties aconitate hydratase in the liver of rats in norm and toxic hepatitis]. Byulleten’ eksperimental’noy biologii i meditsiny. 2004. 4: 399–402. (In Russian).
10. Alekseenko S.A., Shchupak A.Yu., Lebed’ko O.A. Primenenie ursodezoksikholevoy kisloty v kompleksnoy terapii toksicheskikh porazheniy pecheni vsledstvie upotrebleniya spirtsoderzhashchikh dezinfektantov [The use of ursodeoxycholic acid in complex treatment of toxic liver damage due to the use of alcohol-containing disinfectants]. Rossiyskie meditsinskie vesti. 2010; 15 (2): 39–44. (In Russian).
Review
For citations:
Shchupak A.Y., Lebedko O.A., Yukhno V.V. INTEGRAL ESTIMATION OF OXIDATIVE STATUS IN PATIENTS WITH ACUTE TOXIC HEPATITIS AND CHRONIC ALCOHOLIC LIVER DISEASE. Russian Sklifosovsky Journal "Emergency Medical Care". 2016;(1):21-24.
Views:
792
Email this article 